A Caution in the Use of Tritiated Substrates for Monoamine Oxidase Assays |
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| Authors: | Michael E May |
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| Institution: | Division of Endocrinology, Metabolism, and Nutrition, Department of Medicine, Medical University of South Carolina, Charleston, outh Carolina 29403, U.S.A. |
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| Abstract: | Abstract: A monoamine oxidase assay utilizing generally labeled 3H]-serotonin as substrate became nonlinear after only ～5% conversion of initial c.p.m. to product. Subsequent analysis showed that a significant proportion of the tritium label was readily exchangeable into water and that monoamine oxidase activity increased release of label as water. The use of generally labeled substrates for oxidase activities is not recommended. |
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| Keywords: | Monoamine oxidase Serotonin |
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