Isolation and characterization of polymorphic microsatellite loci in Camellia nitidissima Chi (Theaceae) |
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Authors: | Wei Ji-Qing Chen Zong-You Wang Zheng-Feng Tang Hui Jiang Yun-Sheng Wei Xiao Li Xiao-Yi Qi Xiao-Xue |
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Affiliation: | Guangxi Institute of Botany, Guangxi Zhuangzu Autonomous Region and the Chinese Academy of Sciences, Guilin 541006, P.R. China. |
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Abstract: | ? Premise of the study: Microsatellite markers were developed in Camellia nitidissima for further population genetic studies. ? Methods and Results: Eight microsatellite markers were newly developed from C. nitidissima and 7 were transferred from other Camellia species. Two to 13 alleles per locus were identified for these microsatellites. Observed and expected heterozygosities ranged from 0.040 to 0.909, and 0.184 to 0.916, respectively. Four loci showed a significant deviation from Hardy-Weinberg equilibrium and five locus pairs displayed linkage disequilibrium. ? Conclusions: These microsatellite markers will be useful to assess the genetic variation and genetic structure of C. nitidissima. |
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