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Development and characterization of microsatellite loci for Khaya senegalensis (Meliaceae)1
Authors:Sexton G J  Frere C H  Dieters M J  Godwin I D  Prentis P J
Institution:School of Land Crop and Food Sciences, University of Queensland, St Lucia Qld 4072 Australia.
Abstract:? Premise of the study: Microsatellite loci were developed to characterize genetic variation and population subdivision in Khaya senegalensis (Desr.) A. Juss. (Meliaceae). ? Methods and Results: Microsatellite loci were identified from genomic DNA sequences generated using the 454 GS-FLX titanium platform. Primers were designed for 67 tri- and tetranucleotide repeats, of which 20 were selected for 2 multiplexes based on amplification success and band size. Eleven of these loci showed polymorphism in two populations of Khaya senegalensis and amplified in individuals from across the species range. ? Conclusions: These new microsatellite loci will be useful for investigation of the evolutionary and conservation genetics of Khaya senegalensis.
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