| 用ELISA法测定抗人心肌肌球蛋白轻链(CMLC)单克隆抗体的K_D值 |
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| 引用本文： | 杨非易,李平风,葛韵琴. 用ELISA法测定抗人心肌肌球蛋白轻链(CMLC)单克隆抗体的K_D值[J]. 中国生物化学与分子生物学报, 1999, 15(2): 348-350 |
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| 作者姓名： | 杨非易 李平风 葛韵琴 |
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| 作者单位： | 北京医科大学生物化学与分子生物学系 |
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| 摘 要： | 人心肌肌球蛋白由轻链（ｃａｒｄｉａｃｍｙｏｓｉｎｌｉｇｈｔｃｈａｉｎｓ，ＣＭＬＣ）和重链（ｃａｒｄｉａｃｍｙｏｓｉｎｈｅａｖｙｃｈａｉｎｓ，ＣＭＨＣ）组成．当心肌细胞遭到破坏时，ＣＭＬＣ释放入血，可致血中ＣＭＬＣ浓度增高．血中ＣＭＬＣ的测定是一种高度...
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| 收稿时间： | 1999-04-20 |
Enzyme Linked Immunosorbent Assay for K D Value of Anti cardiac Myosin Light Chains (CMLC) Monoclonal Antibodies |
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| YANG Feiyi,LI Pingfeng,GE Yunqin. Enzyme Linked Immunosorbent Assay for K D Value of Anti cardiac Myosin Light Chains (CMLC) Monoclonal Antibodies[J]. Chinese Journal of Biochemistry and Molecular Biology, 1999, 15(2): 348-350 |
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| Authors: | YANG Feiyi LI Pingfeng GE Yunqin |
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| Affiliation: | (Department of Biochemistry and Molecular Biology,Beijing Medical University,Beijing 100083 |
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| Abstract: | A simple ELISA method for characterization of K D value of anti cardiac myosin light chains (CMLC) was developed.CMLC at various concentrations were mixed with a constant amount of CMLC McAb in phosphate buffer.After incubation each mixture was transferred into and incubated in the wells of a microtitration plate previously coated with CMLC, and then reacted with HRP conjugated rabbit antimouse IgG and substrate (TMB) solution.Absorbance was measured at 450 nm. K D values of these three different kinds of CMLC McAb were almost identical,IC 11 D 7:3 9×10 -8 mol/L;2F 6A 4:6 5×10 -8 mol/L and 3F 2G 6:8 0×10 -8 mol/L.ELISA method is a simple and accurate way for characterization of K D value of anti CMLC McAb. |
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| Keywords: | ELISA CMLC McAb K D |
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