Evaluation of the effects of sensory denervation on osteoblasts by 3H-proline autoradiography |
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| Authors: | D J Chiego Jr I J Singh |
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| Institution: | (1) Department of Anatomy, New York University Dental Center, New York, N.Y., USA;(2) Department of Anatomy, New York University Dental Center, 345 E. 24th Street, 10010 New York, N.Y., USA;(3) Present address: Dental Research Institute, University of Michigan, School of Dentistry, 48109 Ann Arbor, Michigan, USA |
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| Abstract: | Summary The inferior alveolar nerve was unilaterally resected in 30-day-old mice; other animals were unilaterally sham-operated. At 15, 30, 60, 90, or 150 days after surgery, the mice were injected with 2 Ci of 3H-proline (sp. act. 1.0 Ci/mM) per g of body weight and killed 15, 30, or 60 min later. Autoradiographs were prepared from 5m decalcified sagittal sections of mandibles and grain counts made over periosteal osteoblasts mesial to the first molar. In denervated mandibles, osteoblasts incorporated less isotope compared to controls with differences being maximal at the early intervals. These differences became attenuated with time, possibly due to an intrinsic compensatory mechanism, secondary to neurotrophic regulation.Supported in part by NSF grant GU-3566 |
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| Keywords: | Autoradiography 3H-proline Osteoblasts Denervation |
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