| 人类胚胎干细胞分化前后CDCA8基因启动子区甲基化状态的实验研究 |
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| 引用本文： | 戴灿,潘艺,苗聪秀,周菂,卢光琇.人类胚胎干细胞分化前后CDCA8基因启动子区甲基化状态的实验研究[J].激光生物学报,2010,19(2):259-262. |
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| 作者姓名： | 戴灿 潘艺 苗聪秀 周菂 卢光琇 |
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| 作者单位： | 中南大学生殖与千细胞工程研究所,人类干细胞国家工程研究中心,湖南,长沙,410078 |
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| 基金项目： | 国家高技术研究发展计划(863计划)，国家重点基础研究发展规划(973计划) |
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| 摘 要： | 目的：分析在人类胚胎干细胞分化过程中,CDCA8基因启动子区甲基化的状态.方法：生物信息学预测人类CDCA8基因上游2 kb区域的CpG岛.抽提未分化和自然分化的人类胚胎干细胞gDNA,应用重亚硫酸盐修饰和DNA序列分析方法检测CDCA8基因启动子区CpG岛甲基化情况.结果：未分化和自然分化的人类胚胎干细胞中,被检测的CDCA8基因启动子区CpC岛均未发现明显的甲基化修饰.结论：在人类胚胎干细胞分化前后,CDCA8基因启动子关键区域的甲基化状态未发生明显改变.
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| 关 键 词： | 胚胎干细胞 CDCA8 甲基化 重亚硫酸盐修饰 |
Investigation of Methylation of CDCA8 Promoter during Differentiation of Human Embryonic Stem Cells |
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| DAI Can,PAN Yi,MIAO Cong-xiu,ZHOU Di,LU Guang-xiu.Investigation of Methylation of CDCA8 Promoter during Differentiation of Human Embryonic Stem Cells[J].ACTA Laser Biology Sinica,2010,19(2):259-262. |
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| Authors: | DAI Can PAN Yi MIAO Cong-xiu ZHOU Di LU Guang-xiu |
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| Institution: | xiu(Institute of Human Reproduction ＆ Stem Cell Engineering National Center of Human Stem Cell Research and Engineering, Central South University . Changsha 410078, Hunan, China) |
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| Abstract: | Objective： To analyze the status of methylation of CDCA8 promoter during differentiation of human embryonic stem cells. Methods ： 2 kb of the 5＇ -flanking region of the human CDCA8 gene were searched for CpG island, gDNA were extracted from both undifferentiated and differentiated human embryonic stern cells, and then were analyzed for methylation of CDCA8 promoter by Bisulfite sequencing PCR. Results： Methylation of CpG islands in the analyzed region of CDCA8 promoter were not detected in both undifferentiated and differentiated human embryonic stem cells. Conclusions ： There were no changes in methylation of CpG islands in the key region of CDCA8 promoter during differentiation of human embryonic stem cells. |
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| Keywords: | CDCA8 |
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