Influence of pH on the UV sensitivity of peritoneal macrophage plasma membrane |
| |
Authors: | Pirutin S K Turovetskii V B Kudryashov Yu B |
| |
Institution: | 1.Biological Faculty, Moscow State University, Moscow, Russia ;2.Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino, 142290, Russia ; |
| |
Abstract: | Lowering the extracellular pH (from 7.2 to 6.3) or intracellular acidification in isolated murine peritoneal macrophages before
UV-irradiating them to 9 J/cm2 (λmax = 306 nm) diminish the percentage of cells with damaged membranes. Extracellular pH 8.4 or intracellular alkalization have
an opposite effect. After transient hypoosmotic swelling, the UV-induced membrane damage is fully pronounced regardless of
external pH. In cells that survive UV-irradiation to 8 and 10 J/cm2 (λmax = 297 nm), the intracellular pH is 0.2 and 0.25 unit lower than in nonirradiated cells. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|