Comparative evaluation of genetic diversity using RAPD,SSR and cytochrome P450 gene based markers with respect to calcium content in finger millet (<Emphasis Type="Italic">Eleusine coracana</Emphasis> L. Gaertn.) |
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Authors: | Preety Panwar Manoj Nath Vijay Kumar Yadav Anil Kumar |
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Institution: | (1) Department of Molecular Biology and Genetic Engineering, G.B. Pant University of Agriculture and Technology, Pantnagar, 263145, India; |
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Abstract: | Genetic relationships among 52 Eleusine coracana (finger millet) genotypes collected from different districts of Uttarakhand were investigated by using randomly amplified
polymorphic DNA (RAPD), simple sequence repeat (SSR) and cytochrome P450 gene based markers. A total of 18 RAPD primers, 10
SSR primers, and 10 pairs of cytochrome P450 gene based markers, respectively, revealed 49.4%, 50.2% and 58.7% polymorphism
in 52 genotypes of E. coracana. Mean polymorphic information content (PIC) for each of these marker systems (0.351 for RAPD, 0.505 for SSR and 0.406 for
cyt P450 gene based markers) suggested that all the marker systems were effective in determining polymorphisms. Pair-wise
similarity index values ranged from 0.011 to 0.999 (RAPD), 0.010 to 0.999 (SSR) and 0.001 to 0.998 (cyt P450 gene based markers)
and mean similarity index value of 0.505, 0.504 and 0.499, respectively. The dendrogram developed by RAPD, SSR and cytochrome
P450 gene based primers analyses revealed that the genotypes are grouped in different clusters according to high calcium (300–450
mg/100 g), medium calcium (200–300 mg/100 g) and low calcium (100–200 mg/100 g). Mantel test employed for detection of goodness
of fit established cophenetic correlation values above 0.95 for all the three marker systems. The dendrograms and principal
coordinate analysis (PCA) plots derived from the binary data matrices of the three marker systems are highly concordant. High
bootstrap values were obtained at major nodes of phenograms through WINBOOT software. Comparison of RAPD, SSR and cytochrome
P450 gene based markers, in terms of the quality of data output, indicated that SSRs and cyt P450 gene based markers are particularly
promising for the analysis of plant genome diversity. The genotypes of finger millet collected from different districts of
Uttarakhand constitute a wide genetic base and clustered according to calcium contents. The identified genotypes could be
used in breeding programmes and amajor input into conservation biology of cereal crops. |
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