Enhanced spore production ofBacillus thuringiensis by fed-batch culture |
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| Authors: | Byung Chul Kang Sang Yup Lee Ho Nam Chang |
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| Affiliation: | (1) BioProcess Engineering Research Center and Department of Chemical Engineering, Korea Advanced Institute of Science and Technology, 305-701 Taeduk Science Town, Taejon, Korea |
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| Abstract: | Summary Fed-batch culture was carried out to increase cell mass followed by batch culture for spore production ofbacillus thuringiensis. High cell mass obtained by increasing the feeding glucose concentration in constant fed-batch culture which supported fast cell growth resulted in good sporulation during subsequent batch culture, and the maximum cell mass of 72.6 g/L and spore concentration of 1.25×1010 spores/mL could be obtained. |
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