A novel replicating circular DNAzyme |
| |
Authors: | Chen Fei Wang Ruijian Li Zhe Liu Bin Wang Xiaoping Sun Yanhong Hao Dongyun Zhang Jin |
| |
Institution: | Key Lab for Molecular Enzymology and Engineering of the Ministry of Education, Jilin University, Changchun, 130023, P.R. China. |
| |
Abstract: | 10–23 DNAzyme has the potential to suppress gene expressions through sequence-specific mRNA cleavage. However, the dependence on exogenous delivery limits its applications. The objective of this work is to establish a replicating DNAzyme in bacteria using a single-stranded DNA vector. By cloning the 10–23 DNAzyme into the M13mp18 vector, we constructed two circular DNAzymes, C-Dz7 and C-Dz482, targeting the β-lactamase mRNA. These circular DNAzymes showed in vitro catalytic efficiencies (kcat/KM) of 7.82 × 106 and 1.36 × 107 M–1·min–1, respectively. Their dependence on divalent metal ions is similar to that found with linear 10–23 DNAzyme. Importantly, the circular DNAzymes were not only capable of replicating in bacteria but also exhibited high activities in inhibiting β-lactamase and bacterial growth. This study thus provides a novel strategy to produce replicating DNAzymes which may find widespread applications. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|