Inhibition of Cyanide-Insensitive Respiration in <Emphasis Type="Italic">Klebsiella oxytoca</Emphasis> SYSU-011 by 8-Hydroxyquinolone |
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| Authors: | Chih Ming Kao You Cheng Hseu Yen Ling Huang Peturs Tang Ssu Ching Chen |
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| Institution: | (1) Institute of Environmental Engineering, National SunYat-Sen University, Kaohsiung, Taiwan, ROC;(2) Department of Cosmeceutics, China Medical University, Taichung, Taiwan, ROC;(3) Department of Biologic Science, National SunYat-Sen University, Kaohsiung, Taiwan, ROC;(4) Bioinformatics Center, Chang Gung University, Taoyuan, Taiwan, ROC;(5) Department of Biotechnology, National Kaohsiung Normal University, Kaohsiung, Taiwan, ROC |
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| Abstract: | The inhibition of the cyanide (KCN)-insensitive respiration of Klebsiella oxytoca SYSU-011 by 8-hydroxyquinoline (8-HQ) was determined. Results showed that the profile of the rate of oxygen uptake of normal-grown
and 8-HQ–grown K. oxytoca SYSU-011 was biphasic and similar, suggesting that 8-HQ did not inhibit the respiration of normal-grown K. oxytoca SYSU-011. A different biphasic KCN inhibition profile of respiration was observed for KCN-grown cells treated with and without
8-HQ. No decrease in respiration rate of KCN-grown cells and a 40% decrease in respiration rate of KCN-grown cells treated
with 8-HQ were observed when KCN concentration was 10–1 mM. Comparing differences of the profiles of oxygen uptake in KCN-grown cells with and without 8-HQ addition indicated that
8-HQ inhibited expression of the KCN-insensitive pathway carried out by nonheme oxidase. Greater inhibition of NADH oxidase
activity by 2-n-heptyl-4-hydroxyquinoline-N-oxide from the cell membrane of the KCN-grown cells treated with 8-HQ, and more H2O2 production from these cells with than without 8-HQ, suggest that the function of the cyanide-insensitive pathway can stabilize
the respiration of the cyanide-grown cells to prevent the production of H2O2. |
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