A simple and rapid method for the purification of the mitochondrial porin from mammalian tissues |
| |
| Authors: | V de Pinto G Prezioso F Palmieri |
| |
| Affiliation: | Department of Pharmaco-Biology, University of Bari, Italy. |
| |
| Abstract: | A new, simple and rapid procedure for the purification of high amounts of mitochondrial porins from different tissues of mammalia is described. The method consists in a single step hydroxyapatite/celite chromatography of Triton X-100 solubilized mitochondrial membranes. For optimal purification several factors are critical such as the absence of salts, a low protein/detergent ratio and an exact hydroxyapatite/celite ratio of 2:1. |
| |
| Keywords: | |
|
|
