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An unusual dependence of human herpesvirus-8 glycoproteins-induced cell-to-cell fusion on heparan sulfate |
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| Authors: | Vaibhav Tiwari Nissar A Darmani Deepak Shukla |
| Institution: | a Department of Ophthalmology, University of Illinois at Chicago, Chicago, IL 60612, USA b Department of Microbiology and Immunology, University of Illinois at Chicago, Chicago, IL 60612, USA c Department of Basic Medical Sciences, College of Osteopathic Medicine of the Pacific and College of Optometry, Western University of Health Sciences, Pomona, CA 91766, USA |
| Abstract: | Human herpesvirus-8 (HHV-8) is known to interact with cell surface heparan sulfate (HS) for entry into a target cell. Here we investigated the role of HS during HHV-8 glycoproteins-induced cell fusion. Interestingly, the observed fusion demonstrated an unusual dependence on HS as evident from following lines of evidence: (1) a significant reduction in cell-to-cell fusion occurred when target cells were treated with heparinase; (2) in a competition assay, when the effector cells expressing HHV-8 glycoproteins were challenged with soluble HS, cell-to-cell fusion was reduced; and, (3) co-expression of HHV-8 glycoproteins gH-gL on target cells resulted in inhibition of cell surface HS expression. Taken together, our results indicate that cell surface HS can play an additional role during HHV-8 pathogenesis. |
| Keywords: | HS heparan sulfate HCjE human conjunctival epithelial cells HHV-8 human herpesvirus-8 |
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