Hematopoietic capacity of preterm cord blood hematopoietic stem/progenitor cells |
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Authors: | Mizue Nakajima Takahiro Ueda Makoto Migita Yuki Oue Takashi Shimada |
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Institution: | a Department of Pediatrics, Nippon Medical School, Tokyo 113-8602, Japan b Department of Biochemistry and Molecular Biology, Nippon Medical School, Tokyo 113-8602, Japan c Department of Pediatrics, Japanese Red Cross Katsushika Maternity Hospital, Tokyo 124-0012, Japan |
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Abstract: | Full-term cord blood (TCB) hematopoietic stem/progenitor cells (HSC/HPCs) are used for stem cell transplantation and are well characterized. However, the properties of preterm cord blood (PCB) HSC/HPCs remain unclear. In the present study, we compared HSC/HPCs from TCB and PCB with respect to their expression of surface markers, homing capacity and ability to repopulate HSCs in the NOD/Shi-scid mice bone marrow. The proportion of CD34+CD38− cells was significantly higher in PCB. On the other hand, the engraftment rate of TCB CD34+ cells into NOD/Shi-scid mice was significantly higher than PCB CD34+ cells. The expression of VLA4 was stronger among TCB CD34+ cells than PCB CD34+ cells. Moreover, there was a positive correlation between the proportion of CD34+CXCR4+ cells and gestational age. These data suggest that the homing ability of HSCs increases during gestation, so that TCB may be a better source of HSCs for transplantation than PCB. |
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Keywords: | Preterm cord blood Hematopoietic stem cells NOD/SCID mice CD34+ cells CXCR4 VLA4 |
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