Fine mapping and comparative genomics integration of two quantitative trait loci controlling resistance to powdery mildew in a Spanish barley landrace |
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Authors: | Cristina?Silvar Email author" target="_blank">Dragan?PerovicEmail author Uwe?Scholz Ana?M?Casas Ernesto?Igartua Frank?Ordon |
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Institution: | 1.Department of Genetics and Plant Production,Aula Dei Experimental Station, CSIC,Zaragoza,Spain;2.Julius Kühn-Institute (JKI), Federal Research Centre for Cultivated Plants, Institute for Resistance Research and Stress Tolerance,Quedlinburg,Germany;3.Leibniz-Institute of Plant Genetics and Crop Plant Research (IPK),Gatersleben,Germany;4.Dpto. de Bioloxía Animal, Bioloxía Vexetal e Ecoloxía,Universidade da Coru?a,A Coru?a,Spain |
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Abstract: | The intervals containing two major quantitative trait loci (QTL) from a Spanish barley landrace conferring broad spectrum
resistance to Blumeria graminis were subjected to marker saturation. First, all the available information on recently developed marker resources for barley
was exploited. Then, a comparative genomic analysis of the QTL regions with other sequenced grass model species was performed.
As a result of the first step, 32 new markers were added to the previous map and new flanking markers closer to both QTL were
identified. Next, syntenic integration revealed that the barley target regions showed homology with regions on chromosome
6 of rice (Oryza sativa), chromosome 10 of Sorghum bicolor and chromosome 1 of Brachypodium distachyon. A nested insertion of ancestral syntenic blocks on Brachypodium chromosome 1 was confirmed. Based on sequence information
of the most likely candidate orthologous genes, 23 new barley unigene-derived markers were developed and mapped within the
barley target regions. The assessment of colinearity revealed an inversion on chromosome 7HL of barley compared to the other
three grass species, and nearly perfect colinearity on chromosome 7HS. This two-step marker enrichment allowed for the refinement
of the two QTL into much smaller intervals. Inspection of all predicted proteins for the barley unigenes identified within
the QTL intervals did not reveal the presence of resistance gene candidates. This study demonstrates the usefulness of sequenced
genomes for fine mapping and paves the way for the use of these two loci in barley breeding programs. |
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