Identification and mapping of two powdery mildew resistance genes in <Emphasis Type="Italic">Triticum boeoticum</Emphasis> L. |
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Authors: | Parveen?Chhuneja Krishan?Kumar Daniel?Stirnweis Severine?Hurni Beat?Keller Harcharan?S?Dhaliwal Email author" target="_blank">Kuldeep?SinghEmail author |
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Institution: | (1) School of Agricultural Biotechnology, Punjab Agricultural University, Ludhiana, 141004, India;(2) Institute of Plant Biology, University of Zurich, Zurich, Switzerland;(3) Present address: Akal School of Biotechnology, Eternal University, Baru Sahib, Himachal Pradesh, 173101, India; |
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Abstract: | Powdery mildew (PM) caused by Blumeria graminis f. sp. tritici (Bgt), is one of the important foliar diseases of wheat that can cause serious yield losses. Breeding for cultivars with diverse
resources of resistance is the most promising approach for combating this disease. The diploid A genome progenitor species
of wheat are an important resource for new variability for disease resistance genes. An accession of Triticum boeoticum (AbAb) showed resistance against a number of Bgt isolates, when tested using detached leaf segments. Inheritance studies in a recombinant inbred line population (RIL), developed
from crosses of PM resistant T. boeoticum acc. pau5088 with a PM susceptible T. monococcum acc. pau14087, indicated the presence of two powdery mildew resistance genes in T. boeoticum acc. pau5088. Analysis of powdery mildew infection and molecular marker data of the RIL population revealed that both powdery
mildew resistance genes are located on the long arm of chromosome 7A. Mapping was conducted using an integrated linkage map
of 7A consisting of SSR, RFLP, STS, and DArT markers. These powdery mildew resistance genes are tentatively designated as
PmTb7A.1 and PmTb7A.2. The PmTb7A.2 is closely linked to STS markers MAG2185 and MAG1759 derived from RFLP probes which are linked to powdery mildew resistance
gene Pm1. This indicated that PmTb7A.2 might be allelic to Pm1. The PmTb7A.1, flanked by a DArT marker wPt4553 and an SSR marker Xcfa2019 in a 4.3 cM interval, maps proximal to PmT7A.2. PmTb7A.1 is putatively a new powdery mildew resistance gene. The powdery mildew resistance genes from T. boeoticum are currently being transferred to cultivated wheat background through marker-assisted backcrossing, using T. durum as bridging species. |
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