Changes in monoamine oxidase activity by mitochondria isolated from late embryos of Bufo bufo |
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| Authors: | A Nicotra O Senatori |
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| Institution: | Dipartimento di Biologia Animale e dell'Uomo, Università di Roma, Italy. |
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| Abstract: | 1. Monoamine oxidase activity has been assayed in mitochondria isolated from post-neural embryos (stages 14-25) of Bufo bufo, using 5-HT and PEA as substrates. 2. Mitochondria isolated from stages 19 to 25 show an increasing ability in deaminating monoamines, PEA being metabolized at a higher level with respect to 5-HT. 3. At all the examined stages 5-HT is metabolized by an enzyme corresponding to MAO A, while PEA, from stage 19, is largely deaminated by a semicarbazide-sensitive amine oxidase (SSAO). 4. The effect of Triton X-100 on MAO A activity appears remarkably different in mitochondria isolated from embryos at stages 14 and 25 respectively. |
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