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Development of a multiplex PCR assay for rapid identification of Burkholderia pseudomallei, Burkholderia thailandensis, Burkholderia mallei and Burkholderia cepacia complex
Authors:Koh Seng Fook  Tay Sun Tee  Sermswan Rasana  Wongratanacheewin Surasakdi  Chua Kek Heng  Puthucheary Savithri D
Institution:Department of Medical Microbiology, Faculty of Medicine, University of Malaya, Malaysia. kohsf.adrian@gmail.com
Abstract:We have developed a multiplex PCR assay for rapid identification and differentiation of cultures for Burkholderia pseudomallei, Burkholderia thailandensis, Burkholderia mallei and Burkholderia cepacia complex. The assay is valuable for use in clinical and veterinary laboratories, and in a deployable laboratory during outbreaks.
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