A sensitive LC-ESI-Q-TOF-MS method reveals novel phytosiderophores and phytosiderophore-iron complexes in barley |
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| Authors: | Tsednee Munkhtsetseg Mak Yit-Wai Chen Yet-Ran Yeh Kuo-Chen |
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| Institution: | Agricultural Biotechnology Research Center, Academia Sinica, Taipei, Taiwan Molecular and Biological Agricultural Sciences, Taiwan International Graduate Program, National Chung-Hsing University and Academia Sinica, Taipei, Taiwan Graduate Institute of Biotechnology and Department of Life Sciences, National Chung-Hsing University, Taichung, Taiwan. |
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| Abstract: | ? The direct analysis of phytosiderophores (PSs) and their metal complexes in plants is critical to understanding the biological functions of different PSs. Here we report on a rapid and highly sensitive liquid chromatography-electrospray ionization-quadrupole-time of flight-mass spectrometry (LC-ESI-Q-TOF-MS) method for the direct and simultaneous determination of free PSs and their ferric complexes in plants. ? In addition to previously reported PSs - deoxymugineic acid (DMA), mugineic acid (MA) and epihydroxymugineic acid (epi-HMA) - two more PSs, avenic acid (AVA) and hydroxyavenic acid (HAVA), were identified by this method in roots of Hordeum vulgare cv Himalaya and in root exudates under iron (Fe) deficiency. ? The two identified PSs could be responsible for Fe acquisition under Fe deficiency because of their relative abundance and ability to form ferric complexes in secreted root exudates. ? This LC-ESI-Q-TOF-MS method greatly facilitates the identification of free PSs and PS-Fe complexes in one plant sample. |
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