七味白术散对菌群失调腹泻小鼠血常规的影响

【目的】探明七味白术散对菌群失调腹泻小鼠血常规的影响情况,为临床应用提供依据。【方法】通过抗生素制作菌群失调腹泻小鼠模型,灌胃给药七味白术散传统汤剂和1/2超微汤剂治疗,采用眼球采血法,用CA-500血液自动分析仪进行测定。【结果】模型组的血小板数、红细胞平均体积和白细胞数较正常组低(P<0.01或P<0.05)。经灌胃治疗后,传统汤剂组和超微汤剂1/2量组的血小板数、红细胞平均体积以及白细胞数在数值上均有所增长,其中传统汤剂组的红细胞平均体积已恢复正常,与正常组比较无统计学差异(P>0.05),超微汤剂1/2量组的血小板数和红细胞平均体积与正常组比较无统计学差异(P>0.05),提示已恢复正常。七味白术散复方能促进血小板、白细胞的生成,对红细胞平均体积有所改善,且超微汤剂的白细胞增加幅度较传统汤剂组大。【结论】七味白术散对菌群失调腹泻小鼠的免疫功能具有一定的调节作用,且超微汤剂1/2量约优于传统汤剂全量。

The effect of Qi-Wei-Bai-Zhu-San on blood of diarrheal mice with dysbacteriosis

Objective] To understand the influence of Qi-Wei-Bai-Zhu-San on blood of diarrheal mice with dysbacteriosis. Methods] Diarrheal mice model with dysbacteriosis was made by feeding antibiotics, then treated with intragastric administration of Qi-Wei-Bai-Zhu-San and 1/2 amount of ultra-micro Qi-Wei-Bai-Zhu-San. The blood was collected by the eyeball method and analyzed with the CA-500 blood automatic instrument. Results] The values of PLT (platelet), MCV (mean corpuscular volume) and WBC (white blood cell) in the model group mice were lowed than that in control group mice (P<0.01 or P<0.05). After treatment by gavage, the values of PLT, MCV and WBC of the traditional decoction group and 1/2 amount of ultra-micro Qi-Wei-Bai-Zhu-San increased, and the MCV of traditional decoction group returned to normal and had no statistical difference comparing with control group (P>0.05). There were no statistics differences in the values of PLT and MCV between ultra-micro 1/2 amount group and control group (P>0.05). It implied that the blood recovered to normal. Qi-Wei-Bai-Zhu-San could promote the production of PLT and WBC, and improve the MCV. The WBC of 1/2 amount of ultra-micro Qi-Wei-Bai-Zhu-San group increased more than that of traditional decoction group. Conclusion] Qi-Wei-Bai-Zhu-San could control immune function of diarrheal mice with dysbacteriosis. The functions of 1/2 amount of ultra-micro Qi-Wei-Bai-Zhu-San were better than that of traditional Qi-Wei-Bai-Zhu-San.