Oxytocin in Leydig cells: an immunocytochemical study of Percoll-purified cells from rat testes |
| |
Authors: | W. S. B. Yeung S. E. F. Guldenaar R. T. S. Worley J. Humphrys B. T. Pickering |
| |
Affiliation: | (1) MRC Neuronal Peptides Research Group, Department of Anatomy, The Medical School, Bristol, UK;(2) Present address: Department of Applied Biology and Chemical Technology, Hong Kong Polytechnic, Hong Kong;(3) MRC Neuronal Peptides Research Group, Department of Anatomy, The Medical School, BS8 1TD Bristol, England, UK |
| |
Abstract: | Summary Testicular interstitial cells, from rats aged 35 days, were dispersed with collagenase and separated through Percoll into 5 fractions (I–V); fraction I being the least dense. Measurement of basal testosterone production, histo-enzymological staining for 3-hydroxysteroid dehydrogenase activity and electron microscopy indicated that the majority of Ley dig cells were found in fraction IV (corresponding to a density of 1.076–1.097 g/ml). In addition, cells from this fraction responded to hCG treatment in a dose-dependent manner on day 0 and remained responsive after being cultured for 1 day. Immunostaining for oxytocin indicated that this fraction also contained the majority of the oxytocin-immunoreactive cells. On day 1 of culture, 56% of the cell population from fraction IV were positively stained for the steroidogenic enzyme and 75% immunoreactive for oxytocin. This overlap indicates that the Leydig cells were also the oxytocin immunoreactive cells. |
| |
Keywords: | Leydig cells Testis 3 /content/p72663222642j23p/xxlarge946.gif" alt=" beta" align=" MIDDLE" BORDER=" 0" >-Hydroxysteroid dehydrogenase Testosterone Oxytocin Rat (Wistar) |
本文献已被 SpringerLink 等数据库收录! |
|