Many monoclonal antibodies with an apparent specificity for certain lung cancers are directed against a sugar sequence found in lacto-N-fucopentaose III |
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| Authors: | L C Huang M Brockhaus J L Magnani F Cuttitta S Rosen J D Minna V Ginsburg |
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| Institution: | 1. National Institute of Arthritis, Diabetes, and Digestive and Kidney Diseases, Bethesda, Maryland 20205 U.S.A.;2. National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20205 U.S.A. |
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| Abstract: | Monoclonal antibodies with an apparent specificity for human small-cell carcinoma, adenocarcinoma, and squamous carcinoma of the lung are produced by some hybridomas obtained from mice and rats immunized with an established line of human small cell lung cancer. Out of 85 of these antibodies produced by independently isolated hybridomas from 15 different fusions, 21 are directed against the sugar sequence which occurs in lacto-N-fucopentaose III ceramide, in several higher glycolipids and in glycoproteins. Specificity was determined by autoradiography of thin-layer chromatograms of glycolipids, by solid-phase radioimmunoassays, and by hapten inhibition studies. All 21 antibodies are of the immunoglobulin M type. |
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| Keywords: | To whom correspondence should be addressed: Building 4 Room 327 National Institutes of Health Bethesda Md 20205 |
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