磷脂组成对脱脂蛋白模型多肽与脂质体相互作用的影响

根据脱脂蛋白的脂结合序列合成了两个两亲性多肽Ａｍｐ１和Ａｍｐ２，在Ａｍｐ２在缬氨酸残基取代了Ａｍｐ１第４位的赖氨酸残基。用内源荧光谱发射峰的蓝移，包埋的钙氯黄素在脂质体中的渗漏，丙烯酰胺对多肽色氨酸残基的淬灭等手段比较了Ａｍｐ１与Ａｍｐ２与具有不同磷脂组成的脂质体的相互作用，并研究了温度的影响。

Influence of Lipid Composition on the Interaction of Apolipoprotein Model Peptides with Liposomes

Two amphiphilic peptides,Ampl and Amp2,were aynthesized according to the sequence of the lipid binding domain in apolipoprotein.Amp2 has a Val residue substituted for the Lys at the 4th position of Ampl.Interactions between Ampl orAmp2 and liposomes with different lipid compositions were compared by studying the blue shifts of the intrinsic fluoresoence emission maxima,the peptide-induced liposome leakage and the quenching of tryptophan fluorescence by acrylamide.The influence of temperature on interactions was Studied as well.Ampl interacted stronger with acidio lipids while Amp2 interacted stronger with zwitterionic lipids. The interaction was reinforced with the increasing extent of lipid unsaturation as well as with the increase of temperature.The lipid unsaturation had a more prominent effect.