人Xp11.2区4.3MbYAC重叠群：大尺度限制图与CpG岛分析

人Ｘｐ１１．２区域具有重要的医学遗传学和基础遗传学价值，它包含很多遗传疾病基因，且至少包含一个逃避Ｘ染色体失活的位点，非常规的基化多态也有发现。我们利用这一区域已知的一系列ＤＮＡ位标，从我们构建的ＹＡＣ库中筛选出一系列ＹＡＣ克隆。

A 4.3-Mb YAC Contig in Human Xp11.2:Long-Range Restriction Mapping and Identification of CpG Islands

The XP 11.2 region of the human X chromosome contains genes involved in a number of inderited diseases, with at least one locus that escapes X chromosome inaetivation,as well as abnormal methylation polymorphism.We isolated a series of yeast artificial chromosome(YAC)clones by hybridization screening with DNA probes localized within this region and assembled them into a 4.3Mb contig spanning from XP 11.21 to XP 11.23 by a combination of Alu-FCR fingerprinting,STS-FCR and DNA probe cross hybridization.On the basis of these overlapping YAC clones we have constructed the long-range restriction map of tnts interval and placed exactly some DNA markers.Four CpG-dense regions between ARAF1 and OATL2 were identified based on the long-range restriction mapping.which indicated the distribution of genes within this interval.It should assist in the future nucleic acid sequence analysis and novel gene identification in this region.