| 嵌合抗体轻链基因在家蚕细胞中的重组与表达 |
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| 引用本文： | 杨冠珍,张文祥.嵌合抗体轻链基因在家蚕细胞中的重组与表达[J].生物化学与生物物理学报,1996,28(6):653-658. |
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| 作者姓名： | 杨冠珍 张文祥 |
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| 作者单位： | [1]中国科学院上海生物化学研究所 [2]上海肿瘤研究所 |
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| 摘 要： | 用家蚕修饰型核多角体病毒为载体，在家蚕细胞获得人鼠嵌合的抗人小细胞肺癌抗体轻链基因的表达。ＰＣＲ和Ｓｏｕｔｈｅｒｎ杂交证明了抗体轻链基因已组建家蚕病毒基因组中。Ｗｅｓｔｅｒｎ ｂｌｏｔ和ＥＬＩＳＡ和分析都检测到在重组病毒感染的家蚕细胞中产生了人鼠嵌合的抗体轻链。
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| 关 键 词： | 家蚕 细胞 嵌合抗体 抗体轻链基因 基因表达 |
The Recombination and Expression of a Chimeric Antibody Light Chain Gene in Cells of Silkworm Bombyx mori |
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| YANG Guan-Zhen and WU Xiang-Fu.The Recombination and Expression of a Chimeric Antibody Light Chain Gene in Cells of Silkworm Bombyx mori[J].Acta Biochimica et Biophysica Sinica,1996,28(6):653-658. |
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| Authors: | YANG Guan-Zhen and WU Xiang-Fu |
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| Abstract: | A chimerio immunoglobulin light-chain gene had been expressed in a baculovirus expression system,which was achieved by infecting the silkworm cells with the recombinant virus r-NPVL2 containing the cDNA of immunoglobulin against a small cell lung cancer.PCR analysis and Southern hybridization proved that the antibody light chain cDNA had been integrated into the modified BmNPV genome.Expression produets of human-mouse chimeric antibody light chain in the silkworm cells were detected by both Western blot and ELISA. |
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| Keywords: | Recombinant BmNPV Chimeric antibody Gene expression |
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