Characterization of two new alleles at the goat CSN1S2 locus |
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| Authors: | L. Ramunno,G. Cosenza,M. Pappalardo,E. Longobardi,D. Gallo,N. Pastore,P. Di Gregorio,& A. Rando |
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| Affiliation: | Dipartimento di Scienze Zootecniche e Ispezione degli Alimenti, Università degli Studi di Napoli Federico II, Napoli, Italy. rammuno@unina.it |
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| Abstract: | Two novel alleles at the goat CSN1S2 locus have been identified: CSN1S2(F) and CSN1S2(D). Sequence analyses revealed that the CSN1S2(F) allele is characterized by a G --> A transition at the 13th nucleotide in exon 3 changing the seventh amino acid of the mature protein from Val to Ile. The CSN1S2(D) allele, apparently associated with a decreased synthesis of alpha s2-casein, is characterized by a 106-bp deletion, involving the last 11 bp of the exon 11 and the first 95 bp of the following intron. Methods (PCR-RFLP and PCR) for identification of carriers of these alleles have been developed. |
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| Keywords: | αs2 casein Capra hircus milk PCR |
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