Hybridization of Gossypium species through in ovulo embryo culture |
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Authors: | Shubhada Thengane S V Paranjpe S S Khuspe A F Mascarenhas |
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Institution: | (1) National Chemical Laboratory, Biochemistry Division, 411 008 Poona, India |
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Abstract: | An interspecific hybrid of the sexually incompatible species G. hirsutum cv. Laxmi and G. arboreum cv. Jyoti was obtained through in ovulo embryo culture. Eightto twelve-day-old ovules were excised and cultured on Beasley and Ting's medium supplemented with Indol-3 acetic acid (5×10-6 to 7×10-6 M), Kinetin (5×10-6 to 5×10-8 M), Gibberellic acid (5×10-7 to 5×10-9M), Ammonium chloride (5 to 15mM) and Casein hydrolysate (50 to 200mg/l) added individually and in various combinations along with sucrose. No single medium was adequate to ensure complete development of the fertilized ovules to plantlets, thus necessitating a sequential five step transfer to different media. Cytological studies confirmed the hybrid nature of the plants.Abbreviation IAA
Indol-3 acetic acid
- Kn
Kinetin
- GA3
Gibberellic acid
- CH
Casein hydrolysate
- NAA
-Naphthalene-acetic acid
- BT
Beasley and Ting's basal medium
- MS
Murashige and Skoog's basal medium
- W
White's basal medium
NCL Communication number 3823. |
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Keywords: | Gossypium interspecific hybrid ovule culture triploid |
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