Methods for peptide identification by spectral comparison |
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Authors: | Jian Liu Alexander W Bell John JM Bergeron Corey M Yanofsky Brian Carrillo Christian EH Beaudrie Robert E Kearney |
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Affiliation: | (1) Center for Cellular & Biomolecular Research, University of Toronto, Toronto, Canada;(2) Montreal Proteomics Network, Montreal, Canada;(3) Department of Anatomy and Cell Biology, McGill University, Montreal, Canada;(4) Department of Biomedical Engineering, McGill University, Montreal, Canada |
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Abstract: | Background Tandem mass spectrometry followed by database search is currently the predominant technology for peptide sequencing in shotgun
proteomics experiments. Most methods compare experimentally observed spectra to the theoretical spectra predicted from the
sequences in protein databases. There is a growing interest, however, in comparing unknown experimental spectra to a library
of previously identified spectra. This approach has the advantage of taking into account instrument-dependent factors and
peptide-specific differences in fragmentation probabilities. It is also computationally more efficient for high-throughput
proteomics studies. |
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