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The effects of detergents DDM and β-OG on the singlet excited state lifetime of the chlorophyll a in cytochrome b6f complex from spinach chloroplasts |
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| Authors: | CHEN XiaoBo ZHAO XiaoHui ZHANG JianPing LI LiangBi KUANG TingYun |
| Affiliation: | 1. Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, Chinese Academy of Sciences,Beijing 100093, China;Graduate University of Chinese Academy of Sciences, Beijing 100039, China 2. State Key Laboratory for Structural Chemistry of Unstable and Stable Species, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100080, China 3. Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, Chinese Academy of Sciences,Beijing 100093, China |
| Abstract: | The singlet excited state lifetime of the chlorophyll a (Chl a) in cytochrome b 6 f (Cyt b 6 f) complex was reported to be shorter than that of free Chl a in methanol, but the value was different for Cyt b 6 f complexes from different sources (∼200 and ∼600 ps are the two measured results). The present study demonstrated that the singlet excited state lifetime is associated with the detergents n-dodecyl-β-D-maltoside (DDM) and n-octyl-β-D-glucopyranoside (β-OG), but has nothing to do with the different sources of Cyt b 6 f complexes. Compared with the Cyt b 6 f dissolved in β-OG, the Cyt b 6 f in DDM had a lower fluorescence yield, a lower photodegradation rate of Chl a, and a shorter lifetime of Chl a excited state. In short, the singlet excited state lifetime, ∼200 ps, of the Chl a in Cyt b 6 f complex in DDM is closer to the true in vivo. |
| Keywords: | cytochrome b6f detergent chlorophyll a singlet excited state lifetime singlet oxygen |
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