<Emphasis Type="Italic">In vitro</Emphasis> micropropagation of four lupin species |
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Authors: | Email author" target="_blank">Tomasz?PniewskiEmail author Józef?Kapusta Andrzej?B?Legocki |
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Institution: | (1) Institute of Bioorganic Chemistry, Polish Academy of Sciences, Noskowskiego 12/14, 61-704 Poznań, Poland |
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Abstract: | The complete protocols for long-term micropropagation of some cultivars of four lupin species: Lupinus luteus, L. albus, L. angustifolius and L. mutabilis were elaborated. The shoots were regenerated in vitro via induction of axillary buds development. Plantlets were multiplicated on lowered salts MS-derived media containing BAP
in diverse and generally low concentrations. Significant differences in regeneration capacity between species and cultivars
were observed. The highest multiplication ratio revealed L. mutabilis and L. luteus. Regenerated shoots were rooted in vitro on low-salts MS-derived media with B5 vitamins. Media were supplemented with different auxins that affected roots formation
of particular species and cultivars. Rooting ability of regenerated shoots decreased rapidly through in vitro culture. For that reason, grafting was applied as an alternative method of transfer of shoots to in vivo conditions. This method turned out to be successful for the majority of studied species and cultivars. Complete rooted or
grafted plantlets were cultivated in pots with perlit in greenhouse.
An erratum to this article is available at . |
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Keywords: | lupin Lupinus L albus L angustifolius L luteus L mutabilis micropropagation rooting grafting |
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