Nested PCR在检测牛白血病病毒前病毒中的应用

用ＮｅｓｔｅｄＰＣＲ检测牛白血病病毒的前病毒形式。从牛白血病病毒基因ｇｐ５１上选择两对引物进行ＮｅｓｔｅｄＰＣＲ体外扩增，产物经２％ａｇａｒｏｓｅ凝胶电泳和用生物素标记的探针鉴定，证实其特异性。结果表明该方法能从两个ＢＡＴ－ＢＬＶ细胞内检测出ＢＬＶ的前病毒ＤＮＡ形式。

Rapid Detection of Proviral DNA of Bovine Leukaemia Virus Using Nested PCR

his paper reported that proviral DNA of bovine leukaemia virus(BLV)was detected bynested PCR.External and internal primers were selected from gp51 gene in BLV genome,Theproducts of the nested PCR were identified by 2?arose 8el electrophoresis and were com-flrmed by hybridlzation with a biotinylated oligonucleotide probe,The results of the nested PCRshow that DNA forms of the provirus were detected from two host cells.