| 莲C0t-1 DNA的荧光原位杂交分析 |
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| 引用本文： | 刁英 佘朝文 胡中立 宋运淳. 莲C0t-1 DNA的荧光原位杂交分析[J]. 广西植物, 2006, 26(5): 459-463 |
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| 作者姓名： | 刁英 佘朝文 胡中立 宋运淳 |
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| 作者单位： | 武汉大学发育生物学教育部重点实验室,湖北,武汉,430072;重庆文理学院生命科学系,重庆,402168;武汉大学发育生物学教育部重点实验室,湖北,武汉,430072 |
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| 基金项目： | 国家科技攻关项目（2002BA546C）;湖南省科技攻关重大项目（2002AA205A）;武汉市科技攻关重点项目（20022005132） |
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| 摘 要： | 为分析中国莲Cot-1DNA在其中期染色体上的分布，从中国莲基因组DNA中分离出Cot-1DNA，将基因组和所分离的Cot-1DNA用生物素标记后作探针，对中国莲染色体进行原位杂交。杂交结果用耦联有荧光素Cy3的生物素抗体检测，发现在每对染色体上均显示出特定的荧光原位杂交带。同时分析了FISH和GISH信号分布的异同。基于Cot-1DNA荧光原位杂交带型及染色体型，构建了中国莲核型。
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| 关 键 词： | 中国莲 C0t-1 DNA 荧光原位杂交 基因组荧光原位杂交 核型 |
| 文章编号： | 1000-3142(2006)05-0459-05 |
| 收稿时间： | 2006-05-11 |
| 修稿时间： | 2006-06-20 |
Study on Cot-1 DNA of Nelumbo nuci f era L. by fluorescence in situ hybridization |
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| DIAO Ying, SHE Chao-wen, HU Zhong-li, SONG Yun-chun. Study on Cot-1 DNA of Nelumbo nuci f era L. by fluorescence in situ hybridization[J]. Guihaia, 2006, 26(5): 459-463 |
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| Authors: | DIAO Ying SHE Chao-wen HU Zhong-li SONG Yun-chun |
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| Affiliation: | 1. Key Laboratory of MOE for Plant Developmental Biology, Wuhan University, Wuhan 430072, China; 2. Department of Life Sciences, Chongqing University of Arts and Sciences, Chongqing 402168, China |
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| Abstract: | In order to study the distribution of C_0t-1 DNA in Nelumbo nucifera,C_0t-1 DNA was extracted from its genomic DNA,labeled with biotin-11-dUTP and in situ hybridized. So did the genomic DNA. The hybridized locations were detected with streptavidin Cy3. Specific fluorescence in situ hybridization(FISH)signals were detected on all individual chromosome pairs. The differences of the signals between C_0t-1 DNA FISH and GISH were discussed. The karyotype has been constructed, on the basis of both C_0t-1 DNA FISH banding patterns and chromosome morphology. |
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| Keywords: | Nelumbo nucifera L. C_0t-1 DNA fluorescence in situ hybridization (FISH) genomic in situ hybridization (GISH) karyotype |
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