| 用单克隆抗体免疫酶染法快速诊断甲乙型流感 |
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| 引用本文： | 陶三菊 刘征利. 用单克隆抗体免疫酶染法快速诊断甲乙型流感[J]. 病毒学报, 1993, 9(1): 100-102 |
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| 作者姓名： | 陶三菊 刘征利 |
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| 作者单位： | 中国预防医学科学院病毒学研究所，中国中医研究院西苑医院，北京市儿童医院儿科研究所，中国预防医学科学院病毒学研究所，中国预防医学科学院病毒学研究所，中国预防医学科学院病毒学研究所，中国中医研究院西苑医院，北京市儿童医院儿科研究所，北京市儿童医院儿科研究所 北京 100052，北京 100052，北京 100052，北京 100052 |
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| 摘 要： |
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| 关 键 词： | 免疫酶技术 单克隆抗体 流行性感冒 |
STUDIES ON RAPID DIAGNOSIS OF INFLUENZA A AND B BY USING IMMUNOENZYME METHOD |
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| Tao Sanju Liu Zhengli Zhaori Getu Yang Dongrong Wang Huanqin Sun Xiaomei Hei Lei Zhang Shuhua Xu YunheInstitute of Virology,CAPM,Beijing,Xi Yuan Hospital,Chinese Academy of Traditional Medicine. STUDIES ON RAPID DIAGNOSIS OF INFLUENZA A AND B BY USING IMMUNOENZYME METHOD[J]. Chinese journal of virology, 1993, 9(1): 100-102 |
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| Authors: | Tao Sanju Liu Zhengli Zhaori Getu Yang Dongrong Wang Huanqin Sun Xiaomei Hei Lei Zhang Shuhua Xu YunheInstitute of Virology CAPM Beijing Xi Yuan Hospital Chinese Academy of Traditional Medicine |
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| Affiliation: | Tao Sanju Liu Zhengli Zhaori Getu Yang Dongrong Wang Huanqin Sun Xiaomei Hei Lei Zhang Shuhua Xu YunheInstitute of Virology,CAPM,Beijing,100052Xi Yuan Hospital,Chinese Academy of Traditional MedicineDepartment of Virology,Beijing Chitdren's Hospital |
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| Abstract: | 100 specimens from out-patients suspected of influenza during early 1991 were collected and inoculated into MDCK celis. The antigen oinfluenza virus in MDCK cell was detected at different time after ino-culation by using immunoenzyme method ( IE ) and immunofluorescence method ( IF ) and as well as conventional virus isolation. Of 100 specimens 16 were found to be positive by IE, in which 12 belonged to type A and 4 belonged to type B. The result of IF was the same with IE. Among 16 specimens positive by IE, 15 isolates were obtained by conventional virus isolation in which 12 isolates were identified as H1N1influenza virus and 3 as influenza B virus by means of hemagglutina-tion inhibition test. No virus can be detected in one specimen by repea-ted isolation. The time needed for diagnosis and typing by IE is about 24-72 hours, whereas it takes about 6-12 days for conventional virus isolation. Therefore, IE method is not only rapid, but also can detect directly the type of inflaenza virus. |
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| Keywords: | Rapid diagnosis Immunoenzyme method Influenza virus Monoclonal antibody |
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