Concentrations of intermediary metabolites in free and calcium alginate-immobilized cells of D-glucose fermenting Saccharomyces cerevisiae

Summary Concentrations of the intracellular intermediary metabolites fructose 1,6-diphosphate, pyruvate, citrate, and malate in free and calcium alginate-immobilized cells of Saccharomyces cerevisiae fermenting D-glucose anaerobically were determined when the sugar up-take rate and the ethanol production rate were constant No cell growth was observed and the fermentation yields and fermentation rates were the same in both types of cells. The concentrations of intermediary intracellular metabolites were also identical for the two types of fermenting cells.