Embryonic stem cells and mice expressing different GFP variants for multiple non-invasive reporter usage within a single animal期刊界 All Journals 搜尽天下杂志传播学术成果专业期刊搜索期刊信息化学术搜索

Embryonic stem cells and mice expressing different GFP variants for multiple non-invasive reporter usage within a single animal

Authors:	Anna-Katerina?Hadjantonakis author-information" > author-information__contact u-icon-before" > mailto:akh@columbia.edu" title=" akh@columbia.edu" itemprop=" email" data-track=" click" data-track-action=" Email author" data-track-label=" " >Email author,Suzanne?Macmaster,Andras?Nagy

Affiliation:	(1) Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Canada;(2) Department of Molecular and Medical Genetics, University of Toronto, Toronto, Canada;(3) Department of Genetics and Development, Columbia University, New York, USA

Abstract:	Background Non-invasive autofluorescent reporters have revolutionized lineage labeling in an array of different organisms. In recent years green fluorescent protein (GFP) from the bioluminescent jellyfish Aequoria Victoria has gained popularity in mouse transgenic and gene targeting regimes [1]. It offers several advantages over conventional gene-based reporters, such as lacZ and alkaline phosphatase, in that its visualization does not require a chromogenic substrate and can be realized in vivo. We have previously demonstrated the utility and developmental neutrality of enhanced green fluorescent protein (EGFP) in embryonic stem (ES) cells and mice [2].

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