A continuous-flow automated assay for iodometric estimation of hydroperoxides |
| |
| Authors: | S M Thomas W Jessup J M Gebicki R T Dean |
| |
| Institution: | Department of Biology/Biochemistry, Brunel University, Uxbridge, Middlesex, England. |
| |
| Abstract: | An iodometric method for the analysis of hydroperoxides has been automated to allow analysis of aqueous biological samples (containing less than 20 mg/ml protein) and lipid hydroperoxide extracts. The evolution of triiodide ions is measured spectrophotometrically at 360 nm. Dependent on the type of sample, 30-60 samples can be analyzed per hour and the system allows detection of less than 100 pmol of peroxide. The assay is linear over a range of 100 pmol to 25 nmol. The sample volume used routinely was 80 microliters. |
| |
| Keywords: | |
|
|
