Human pituitary growth hormone: immunochemical investigations of biologically active fragments |
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Authors: | W C Clarke T Hayashida C H Li |
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Institution: | Hormone Research Laboratory, University of California, San Francisco, California 94143 U.S.A. |
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Abstract: | Guinea pig antisera to human growth hormone were tested for their ability to recognize the two biologically active fragments of the hormone produced by human plasmin digestion and a synthetic active fragment. A precipitin line was obtained with native human growth hormone, plasmin-treated human growth hormone, and its NH2-terminal fragment (residues 1–134). In the microcomplement-fixation and radioimmunoassay experiments, the NH2-terminal plasmin fragment (residues 1–134) showed a greater immunoreactivity than the COOH-terminal plasmin fragment (residues 141–191). This, in turn, was more active than the synthetic fragment (residues 95–136). |
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