Activity enhancement and stabilization of lipase from Pseudomonas cepacia in polyallylamine-mediated biomimetic silica |
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Authors: | Guan-Chun Chen I-Ching Kuan Jian-Ren Hong Bing-Hong Tsai Shiow-Ling Lee Chi-Yang Yu |
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Affiliation: | Department of Bioengineering, Tatung University, 40 Chungshan N. Rd. Sec. 3, Taipei, 10452, Taiwan. |
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Abstract: | Triacylglycerol lipase from Pseudomonas cepacia and Fe(3)O(4) magnetic nanoparticles were encapsulated simultaneously within biomimetic silica through the catalysis of polyallylamine. The encapsulation efficiency reached 96% with an activity recovery of 51%. After 5 h at 37°C, the activities of the free and encapsulated lipases decreased by 77 and 16%, respectively. Addition of 10 and 15 mol% trimethylmethoxysilane to tetramethoxysilane during encapsulation doubled the lipase activity while inclusion of 50 and 60 mol% γ-(methacryloxypropyl)-trimethoxysilane tripled the activity. Thus, such encapsulation not only stabilized P. cepacia lipase but also could enhance the activity by varying silane additives. |
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