Sequence-specific assignment of the backbone 1H-and 31P-NMR lines in a short DNA duplex with homo- and heteronuclear correlated spectroscopy |
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| Authors: | M. H. Frey,W. Leupin,O. W. S rensen,W. A. Denny,R. R. Ernst,K. Wü thrich |
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| Affiliation: | M. H. Frey,W. Leupin,O. W. Sørensen,W. A. Denny,R. R. Ernst,K. Wüthrich |
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| Abstract: | We report the assignment of the backbone 1H- and 31P-nmr lines in the synthetic hexadeoxyribonucleotide pentaphosphate duplex d(GCATGC)2, using double quantum filtered 1H-1H correlation spectroscopy, 1H observed 1H-31P heteronuclear correlation spectroscopy, and 31P relayed 1H-1H correlation spectroscopy. The strategy used enables one to make sequence-specific resonance assignments without reference to a known or assumed conformation of the DNA fragment. |
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