A specific method for the determination of soluble sugars in plant extracts using enzymatic analysis and its application to the sugar content of developing pear fruit buds |
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| Authors: | C A Blunden M F Wilson |
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| Institution: | 1. Advanced Biotechnology Center, Dubai – 181499, United Arab Emirates;2. Laboratory for Food Chemistry & Microbiology, Department of Food and Nutrition Science, Pavillon Paul comtois, Universite Laval, Quebec G1K7P4, Canada;3. CAS in Botany, University of Madras, Guindy Campus, Chennai, India;4. Alka Research Foundation, Anna Nagar, Maruthamalai Adivaaram, Coimbatore, Tamilnadu, India;1. Key Laboratory of Agrometeorology of Jiangsu Province, Institute of Ecology, School of Applied Meteorology, Nanjing University of Information Science & Technology, Nanjing 210044, China;2. Hokkaido Research Center, Forestry and Forest Products Research Institute (FFPRI), Forest Research and Management Organization, 7 Hitsujigaoka, Sapporo, Hokkaido 062-8516, Japan;3. Department of Public Health, Environmental Health Sciences, Morrill I, N344, University of Massachusetts, Amherst, MA 01003, USA |
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| Abstract: | A simple manual method for the routine analysis of glucose, fructose, mannose, galactose, and sucrose in plant material is described. The technique is demonstrated using various seeds known to form part of the diet of the bullfinch (Pyrrhula pyrrhula L.), a pest of commercial orchards in southeast England. The method involves the overnight extraction of the sugars with 62.5% aqueous methanol, followed by the conversion of the individual sugars to gluconate 6-phosphate (or, in the case of galactose, to galactono-gamma-lactone) by specific enzymes, and their determination spectrophotometrically. |
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