Supported phospholipid bilayers for two-dimensional protein crystallization |
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| Authors: | E E Uzgiris |
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| Affiliation: | 1. Division for Organic Chemistry and Biochemistry, Ruđer Bošković Institute, Bijenička 54, 10000 Zagreb, Croatia;2. Division for Physical Chemistry, Ruđer Bošković Institute, Bijenička 54, 10000 Zagreb, Croatia;3. Department of Mathematics, University of Chemistry and Technology, 166 28 Prague, Czech Republic;1. College of Food Science, Southwest University, Chongqing 400715, PR China;2. Cooperative Innovation Center of Lipid Resources and Children''s Daily Chemicals, Chongqing University of Education, Chongqing 400067, PR China |
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| Abstract: | Phospholipid bilayers, supported on UV irradiated carbon shadowed nitrocellulose electron microscope grids, have been used to induce two-dimensional crystal growth of IgE and IgG anti-DNP monoclonal antibodies. The UV irradiation renders the grids hydrophilic in a very uniform fashion and allows for the transfer of phospholipid monolayers from an air/water interface in a sequential dipping procedure. The surface coverage achieved was nearly 100% as measured by antibody binding and by the formation of protein arrays on the bilayer covered grids. The supported bilayers appear to be stably held and are appropriate for slow binding conditions and long incubation times with low concentrations of binding protein. |
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