Occludin expression in goldfish held in ion-poor water |
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Authors: | Helen Chasiotis Jennifer C Effendi Scott P Kelly |
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Institution: | (1) Department of Biology, York University, 4700 Keele Street, Toronto, ON, M3J 1P3, Canada |
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Abstract: | With an emphasis on the tight junction protein occludin, the response of goldfish following abrupt exposure (0–120 h) as well
as long-term acclimation (14 and 28 days) to ion-poor water (IPW) was examined. Both abrupt and long-term exposure to IPW
lowered serum osmolality, Na+] and Cl−], and elevated serum glucose. After abrupt exposure to IPW, gill tissue exhibited a prompt and sustained decrease in Na+–K+–ATPase activity, and a transient increase in occludin expression that returned to control levels by 6 h. Following 14 and
28 days in IPW, gill occludin expression was markedly elevated, while Na+–K+–ATPase activity was only significantly different (elevated) at day 14. Kidney tissue exhibited an elevation in both Na+–K+–ATPase activity and occludin expression after 28 days; however, in the intestine, occludin expression declined at day 14
but did not differ from FW fish at day 28. These studies demonstrate that goldfish can tolerate abrupt as well as sustained
exposure to ion-poor surroundings. Data also suggests that occludin may play an adaptive role in fishes acclimated to ion-poor
conditions by contributing to the modulation of epithelial barrier properties in ionoregulatory tissues.
Helen Chasiotis and Jennifer C. Effendi contributed equally to this work. |
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Keywords: | Tight junction Gill Kidney Paracellular permeability Osmoregulation |
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