Occurrence of introns in the 16S rRNA genes of members of the genus Thermoproteus |
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Authors: | T Itoh Ken-ichiro Suzuki Takashi Nakase |
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Institution: | (1) Japan Collection of Microorganisms, The Institute of Physical and Chemical Research (RIKEN), 2-1 Hirosawa, Wako-shi, Saitama 351-0198, Japan e-mail: ito@ulmus.riken.go.jp Tel.: +81-48-467-9560; Fax: +81-48-462-4617, JP |
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Abstract: | Multiple introns were detected in the 16S rRNA gene of newly isolated Thermoproteus species strains IC-033 and IC-061 and Thermoproteus neutrophilus JCM 9278. In the 16S rRNA gene of strain IC-033, five introns of 627, 762, 636, 33, and 682 bp existed after positions 548,
781, 1092, 1205, and 1213 (according to the Escherichia coli numbering system), respectively. Likewise, strain IC-061 possessed 764-, 32-, and 688-bp introns after positions 781, 1205,
and 1213, respectively; and T. neutrophilus JCM 9278 had 34- and 663-bp introns after positions 1205 and 1213, respectively. All the introns carried the putative intron
core structures consisting of a bulge-helix-bulge motif and a long stable stem. The large introns carried open reading frames
containing the LAGLI-DADG-like motifs in their terminal inserts; however, three out of four large introns of strain IC-033
seemed to incur frameshift mutations. Occurrence of introns at the same insertion sites in the three strains would allow tracing
of the evolutionary movements of these introns.
Received: 16 February 1998 / Accepted: 27 April 1998 |
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Keywords: | Archaea Hyperthermophile Thermoproteus Intron 16S rRNA gene LAGLI-DADG-like motif |
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