Eumenitin, a novel antimicrobial peptide from the venom of the solitary eumenine wasp Eumenes rubronotatus |
| |
Authors: | Konno Katsuhiro Hisada Miki Naoki Hideo Itagaki Yasuhiro Fontana Renato Rangel Marisa Oliveira Joacir Stolarz Cabrera Marcia Perez Dos Santos Neto João Ruggiero Hide Izumi Nakata Yoshihiro Yasuhara Tadashi Nakajima Terumi |
| |
Affiliation: | Center for Applied Toxinology, Butantan Institute, S?o Paulo, SP 05503-900, Brazil. kk-gon@butantan.gov.br |
| |
Abstract: | A novel antimicrobial peptide, eumenitin, was isolated from the venom of the solitary eumenine wasp Eumenes rubronotatus. The sequence of eumenitin, Leu-Asn-Leu-Lys-Gly-Ile-Phe-Lys-Lys-Val-Ala-Ser-Leu-Leu-Thr, was mostly analyzed by mass spectrometry together with Edman degradation, and corroborated by solid-phase synthesis. This peptide has characteristic features of cationic linear alpha-helical antimicrobial peptides, and therefore, can be predicted to adopt an amphipathic alpha-helix secondary structure. In fact, the CD spectra of eumenitin in the presence of TFE or SDS showed a high content of alpha-helical conformation. Eumenitin exhibited inhibitory activity against both Gram-positive and Gram-negative bacteria, and moderately stimulated degranulation from the rat peritoneal mast cells and the RBL-2H3 cells, but showed no hemolytic activity against human erythrocytes. This antimicrobial peptide in the eumenine wasp venom may play a role in preventing potential infection by microorganisms during prey consumption by their larvae. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|