Intracellular distribution of enzymes associated with nitrogen assimilation in roots |
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Authors: | A. Suzuki P. Gadal A. Oaks |
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Affiliation: | (1) Laboratoire de Biologie Végétale, ERA au CNRS No 799, Faculté des Sciences, Université de Nancy I, Case Officielle no 140, F-54037 Nancy Cedex, France;(2) Biology Department, McMaster University, L8S 4KI Hamilton, Ontario, Canada |
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Abstract: | The cellular distribution of enzymes involved in nitrogen assimilation: nitrate reductase (EC 1.6.6.2), nitrite reductase (EC 1.6.6.4), glutamine synthetase (EC 6.3.1.2), glutamate synthase (EC 2.6.1.53), and glutamate dehydrogenase (EC 1.4.1.3) has been studied in the roots of five plants: maize (Zea mays L. hybrid W 64A x W 182E), rice (Oryza sativa L. cv. Delta), bean (Phaseolus vulgaris L. cv. Contender), pea (Pisum sativum L. cv. Demi-nain), and barley (Hordeum vulgare L.). Initially, cell organelles were separated from soluble proteins by differential centrifugation. Cell organelles were also subjected to sucrose density gradients. The results obtained by these two methods indicate that nitrite reductase and glutamate synthase are localized in plastids, nitrate reductase and glutamine synthetase are present in the cytosol, and glutamate dehydrogenase is a mitochondrial enzyme. |
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Keywords: | Nitrogen assimilation enzymes Organelles, enzyme distribution Root |
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