LH-producing cells in the ovine pituitary |
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Authors: | Dr F Dacheux M P Dubois |
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Institution: | (1) Laboratoire de Physiologie Comparée, Faculté des Sciences, Tours, France;(2) I.N.R A. — Physiologie Reproduction, 37380 Nouzilly, France;(3) I.N.R.A. — Physiologie de la Reproduction, Nouzilly, France |
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Abstract: | Summary An immunocytochemical technique using the peroxidase-antiperoxidase complex (PAP) was applied to identify and characterize the LH-secreting cells in the ovine pituitary at the ultrastructural level. These cells, round or oval in shape, possessing flattened cisternae of the rough endoplasmic reticulum, contain one class of secretory granules (mean diameter 250 nm) and large dense bodies (600 to 800 nm in diameter). LH molecules and the two subunits LH and LH were localized on the secretory granules and on the small granules near the Golgi complex. The large dense bodies, the cisternae of the endoplasmic reticulum and the saccules of the Golgi complex showed no reaction product.Abbreviations used in this Article O-LH
ovine luteinizing hormone
- b-LH
bovine luteinizing hormone
- p-LH
porcine luteinizing hormone
- p-LH
porcine LH subunit
- p-LH
porcine LH subunit
- O-FSH
ovine follicle stimulating hormone
- b-TSH
bovine thyrotropic hormone
- A-b LH
antiserum to bovine LH
- A-pLH
antiserum to porcine LH subunit
- A-pLH
antiserum to porcine LH subunit |
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Keywords: | Anterior pituitary (ovine) Gonadotropic cells Electron microscopic immunocytochemistry |
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