Chromosome segregation in an asymmetrically dividing bacterium,Caulobacter crescentus |
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Authors: | Hideo Iba Yoshimi Okada |
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Affiliation: | Department of Biophysics and Biochemistry Faculty of Science, University of Tokyo Hongo, Tokyo 113, Japan |
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Abstract: | The mode of chromosome segregation in an asymmetrically dividing bacterium, Caulobacter crescentus, was studied by examining the fate of labeled DNA strands. Swarmer cells (one type of Caulobacter daughter cell), in which single strands of DNA had been labeled with [3H]thymidine during the previous round of chromosome replication, were grown synchronously in a non-radioactive medium for two generations. The distribution of radioactivity among the cells was visualized by autoradiography under a phase-contrast microscope. The labeled DNA strands in each cell were found to consist of two conserved units. From this, we propose a model in which the swarmer cell has two identical chromosomes, which are segregated into the progeny swarmer cell and the progeny stalked cell after chromosome replication. |
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Keywords: | Author to whom correspondence should be addressed. |
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