An IL-6/IL-6 soluble receptor (IL-6R) hybrid protein (H-IL-6) induces EPO-independent erythroid differentiation in human CD34(+) cells |
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Authors: | Baiocchi M Marcucci I Rose-John S Serlupi-Crescenzi O Biffoni M |
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Affiliation: | Istituto Ricerca Cesare Serono S.p.A, Via di Valle Caia 22, 00040, Ardea, (RM), Italy. mbaiocchi@uniroma1.it |
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Abstract: | H-IL-6 is a hybrid protein constructed to contain IL-6 and its soluble receptor linked by a flexible peptide chain. Here we show that H-IL-6 strongly enhances proliferation of human CD34(+)cells in serum-free liquid culture, and that the majority of the cells generated belong to the erythroid lineage, being positive for the marker Glycophorin A. Conversely, H-IL-6 does not increase the number of myeloid, CD13-positive cells. Comparable effects are observed on progenitors from cord blood and adult peripheral blood. Therefore, H-IL-6 triggers an erythroid-inducing signal in haematopoietic progenitor cells, independently from erythropoietin (EPO). |
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