Culture of human pituitary prolactin and growth hormone cells |
| |
| Authors: | J Snyder Dr W C Hymer W W Wilfinger |
| |
| Institution: | (1) Department of Biochemistry and Biophysics, The Pennsylvania State University, University Park, Pennsylvania, USA;(2) 401 Althouse Laboratory, Penn State University, 16802 University Park, PA, USA;(3) Present address: Department of Biochemistry, University of Texas, Health Science Center at Dallas, 73235 Dallas, Texas, USA;(4) Present address: Department of Physiology, University of Cincinatti, Medical School, 45221 Cincinatti, Ohio, USA |
| |
| Abstract: | Summary Fragments of pituitary tissue obtained from a total of 37 patients with either breast cancer, diabetic retinopathy, galactorrhea, or acromegaly were dissociated into single cell suspensions prior to cell culture. Release of human growth hormone (hGH) and human prolactin (hPRL) into the culture medium was measured by radioimmunoassay. During a 3-week culture period, prolactin cells released 9–13 times the intracellular levels of hPRL at the time of seeding, whereas hGH release from growth hormone cells was only 1–2 times that of their initial intracellular level during this same time. Both growth hormone and prolactin cells retained distinctive ultrastructural features during culture. The prolactin cells responded to TRH stimulation by elevated release of PRL into the medium. No evidence for mitotic division of prolactin cells in vitro was found.This work was supported by NCI Contract NO 1-CB-23863 |
| |
| Keywords: | Pituitary Human Cell culture Prolactin Growth hormone |
| 本文献已被 SpringerLink 等数据库收录！ |
|
