An Efficient Rice Transformation System Utilizing Mature Seed-derived Explants and a Portable,Inexpensive Particle Bombardment Device |
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Authors: | Sudhakar Durailagaraja Duc Le Tan Bong Bui Ba Tinjuangjun Porntip Maqbool Shahina Bano Valdez Marta Jefferson Richard Christou Paul |
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Abstract: | We developed a practical and efficient gene transfer system for indica rice utilizing mature-seed derived explants and a simple bombardment device which uses compressed helium for accelerating DNA-coated metal particles. Unlike instruments which have been described in the literature previously, this new bombardment device, which is an improvement of the particle inflow concept, does not require vacuum. This attribute simplifies the transformation procedure significantly and it makes rice transformation technology accessible to laboratories which may not have the resources to invest in more expensive particle bombardment instruments. We determined experimentally that we could recover transgenic rice plants utilizing three different particle bombardment instruments at comparable frequencies. |
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Keywords: | Oryza sativa particle bombardment transformation transgenic rice |
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